Abstract: This research aims to study the influence of NLP training program in reducing test anxiety by analyzing biological feedback. This empirical study assessed pre- and post-tests of a study sample consisting of 30 students aged 19.6 years that were diagnosed with high test anxiety. The students were divided into an experimental group and a control group; each with 15 students. The experimental group was trained on 24 units of NLP strategies for 3 months, i.e. 2 units per week with each unit lasting for 40-60 minutes. The anxiety test scale was applied on par with observations of vital biological changes (heartrate and blood pressure). The results showed statistically significant differences in the average scores of test anxiety before and after applying the aforementioned program mean ± SD 198 ± 11.9 (P< 0.05) for the post-test results were better than the pre-test ones. Moreover, there were statistically significant differences between the experimental and control groups indicating better results achieved by the experimental group when they took the post-test. In sum, the NLP program had a highly positive influence in reducing test anxiety and undesirable biological changes when applied to the experimental group.